期刊
PHYTOMEDICINE
卷 19, 期 8-9, 页码 756-762出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.phymed.2012.02.014
关键词
Cell cycle; Colon-rectal cancer; Triptolide; Tripterygium wilfordii; p21
资金
- National Natural Science Foundation of China [81071689]
- pre-973 national basic research program [2010CB535002]
- Special Key New Drug Development Technology Project [2009ZX09103]
- Shaanxi Foundation of Technology [201010 4-02-07]
- Research Foundation for the Returned Overseas Scholars in Fourth Military Medical University
Triptolide, a diterpene triepoxide compound extracted from the traditional Chinese medicine herb Tripterygium wilfordii Hook F., is a potential cancer chemotherapeutic for tumors. However, the mechanism of anti-proliferative mechanism of triptolide in colon cancer cells is not entirely clear. Triptolide markedly inhibited HT29 and SW480 cells proliferation in a dose- and time-dependent manner. Triptolide decreased ERR and ART phosphorylation, and GABP alpha expression in colon cancer cells. Beta-catenin expression and phosphorylation were not altered by incubation of triptolide. However, we found that triptolide repressed expression of LEF/TCF. Although it did not significantly affect cells apoptosis, triptolide induced G1 phase arrest dose-dependently. Further detection for the expression of cell cycle-related proteins suggesting that triptolide stimulate expression of p21 and repress cyclin Al. Increased p21 binded to CDK4/CDK6, therefore blocked function of CDK4/CDK6, and subsequently contribute to the G1 arrest. These data suggested that triptolide is a potential agent for treatment of colon cancer, and its anti-proliferation effect mainly occur through G1 phase arrest. (C) 2012 Elsevier GmbH. All rights reserved.
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