期刊
PHYTOMEDICINE
卷 18, 期 11, 页码 934-940出版社
ELSEVIER GMBH
DOI: 10.1016/j.phymed.2011.02.007
关键词
Rhodomyrtone; Rhodomyrtus tomentosa; Glycolysis; Proteomics; Streptococcus pyogenes; Two-dimensional gel electrophoresis
资金
- Thailand Research Fund
- Royal Golden Jubilee [PHD/0029/2548]
- Thailand's Office of the Higher Education Commission
- Van Leersumfonds [VLF/DA/3689]
- CEU [LSHM-CT-2006-019064, LSHG-CT-2006037469]
Rhodomyrtone from Rhodomyrtus tomentosa (Aiton) Hassk. leaf extract has a strong antibacterial activity against the bacterial pathogen Streptococcus pyogenes. Our previous studies indicated that the bactericidal activity of rhodomyrtone might involve intracellular targets. In the present studies we followed a proteomics approach to investigate the mode of action of rhodomyrtone on S. pyogenes. For this purpose, S. pyogenes was cultivated in the presence of 0.39 mu g/ml rhodomyrtone, which corresponds to 50% of the minimal inhibitory concentration. The results show that the amounts of various enzymes associated with important metabolic pathways were strongly affected, which is consistent with the growth-inhibiting effect of rhodomyrtone. Additionally, cells of S. pyogenes grown in the presence of rhodomyrtone produced reduced amounts of known virulence factors, such as the glyceraldehyde-3-phosphate dehydrogenase, the CAMP factor, and the streptococcal pyrogenic exotoxin C. Taken together, these findings indicate that rhodomyrtone has both antimicrobial and anti-infective activities, which make it an interesting candidate drug. (C) 2011 Elsevier GmbH. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据