期刊
PHYTOMEDICINE
卷 16, 期 2-3, 页码 233-243出版社
ELSEVIER GMBH
DOI: 10.1016/j.phymed.2008.08.006
关键词
Grape seed proanthocyanidins; Metabolism; LC-MS/MS; Brain
资金
- National Center for Complementary and Alternative Medicine
- National Institutes of Health Office of Dietary Supplements [5P50 AT-00477]
- UAB Alzheimer Disease Research Center [1P50AG16582-08]
- UAB Memory Disorder Research center
- NIH/NCRR Shared Instrumentation [S10 RR19231]
- NCI [P30 CA13148]
- NATIONAL CANCER INSTITUTE [P30CA013148] Funding Source: NIH RePORTER
- NATIONAL CENTER FOR COMPLEMENTARY &ALTERNATIVE MEDICINE [P50AT000477] Funding Source: NIH RePORTER
- NATIONAL CENTER FOR RESEARCH RESOURCES [S10RR019231] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [P50AG016582] Funding Source: NIH RePORTER
Proanthocyanidin rich plant extracts derived from grape seed extract (GSE), hawthorn and cranberry are on markets for their preventive effects against cardiovascular diseases and uroinfections in woman. However, the importance of these health beneficial effects of these botanicals remains elusive due to incomplete understanding of uptake, metabolism and bioavailability of proanthocyanidins in vivo. In the present study rats were given GSE orally (300 mg/kg, twice a day) and blood and urine were collected over a 24h period. Monomeric catechins and their methylated metabolites, and proanthocyanidins up to trimers were detected in blood samples treated with GSE using LC-MS/MS operating in the multiple reaction monitoring (MRM) mode. A new tetramethylated metabolite of dimeric proanthocyanidin (m/z 633) in GSE-treated urine was tentatively identified. Using LC-MS/MS, (+)-catechin and (-)epicatechin were identified in the brain conclusively. These data suggested that GSE catechins cross the blood brain barrier and may be responsible for the neuroprotective effects of GSE. (C) 2008 Elsevier GmbH. All rights reserved.
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