期刊
PHYTOCHEMISTRY
卷 100, 期 -, 页码 34-42出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2014.01.017
关键词
Snow alga; Chloromonas pichinchae; Lipidomics; ESI; Triacylglycerols; Silver-LC/APCI-MS; NARP-LC/APCI-MS
资金
- project GACR [14-00227S]
- Institutional Internal Project [RVO61388971]
The main analytical benefit of this study is the development of methods enabling a rapid determination of total lipids of algae by lipidomic analysis and detailed identification and quantification of a complex mixture of natural TAGs by silver-LC/APCI-MS and NARP-LC/APCI-MS. Both types of chromatography can readily identify, both qualitatively and semiquantitatively, triacylglycerols containing 16:3 and 16:4 acids in the molecule. We conclude that the genus Chloromonas is a major producer of C16 PUFAs mostly contained in TAGs. Since more detailed studies in this field have been stymied by the shortage of 16:3 and 16:4 FAs, we decided to study the alga Chloromonas as a potential biotechnological source of C16 PUFAs. (C) 2014 Elsevier Ltd. All rights reserved.
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