期刊
PHYTOCHEMISTRY
卷 101, 期 -, 页码 23-31出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2014.02.009
关键词
Madagascar Periwinkle; Catharanthus roseus; Apocynaceae; Secologanin biosynthesis; 7-Deoxyloganetic acid synthase; Virus induced gene silencing; Functional characterization
资金
- National Sciences and Engineering Research Council of Canada
- Canada Research Chair in Plant Biotechnology
- Genome Canada
- Genome Alberta
- Genome Prairie
- Genome Quebec
- Genome British Columbia
- Canada Foundation for Innovation
- Ontario Ministry of Research and Innovation
- National Research Council of Canada
Iridoids are key intermediates required for the biosynthesis of monoterpenoid indole alkaloids (MIAs), as well as quinoline alkaloids. Although most iridoid biosynthetic genes have been identified, one remaining three step oxidation required to form the carboxyl group of 7-deoxyloganetic acid has yet to be characterized. Here, it is reported that virus-induced gene silencing of 7-deoxyloganetic acid synthase (7DLS. CYP76A26) in Catharanthus roseus greatly decreased levels of secologanin and the major MIAs, catharanthine and vindoline in silenced leaves. Functional expression of this gene in Saccharomyces cerevisiae confirmed its function as an authentic 7DLS that catalyzes the 3 step oxidation of iridodial-nepetalactol to form 7-deoxyloganetic acid. The identification of CYP76A26 removes a key bottleneck for expression of iridoid and related MIA pathways in various biological backgrounds. (C) 2014 Elsevier Ltd. All rights reserved.
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