期刊
PHYTOCHEMISTRY
卷 95, 期 -, 页码 242-251出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2013.08.008
关键词
Dendrobium denneanum; Orchidaceae phenanthrene; Anti-inflammatory activity; iNOS; NF-kappa B; MAPKs; Cytotoxic; RAW264.7 cell
资金
- National New Drug Innovation Major Project of China [2011ZX09307-002-02]
- Key Projects in the National Science & Technology Pillar Program [2011BAC09B04]
- Knowledge Innovation Program of the Chinese Academy of Sciences [XBCD-2011-007]
- Twelfth Five-Year Chinese Herbal Medicines Breeding Research of Sichuan Province
Cultivated Dendrobium denneanum has been substituted for other endangered Dendrobium species in recent years, but there have been few studies regarding either its chemical constituents or pharmacological effects. In this study, three phenanthrene glycosides, three 9,10-dihydrophenanthrenes, two 9,10-dihydrophenanthrenes glycosides, and four known phenanthrene derivatives, were isolated from the stems of D. denneanum. Their structures were elucidated on the basis of MS and NMR spectroscopic data. Ten compounds were found to inhibit nitric oxide (NO) production in lipopolysaccharide (LPS)-activated mouse macrophage RAW264.7 cells with IC50 values of 0.7-41.5 mu M, and exhibited no cytotoxicity in RAW264.7, HeLa, or HepG2 cells. Additionally, it was found that 2,5-dihydroxy-4-methoxy-phenanthrene 2-O-beta-D-glucopyranoside, and 5-methoxy-2,4,7,9S-tetrahydroxy-9,10-dihydrophenanthrene suppressed LPS-induced expression of inducible NO synthase (iNOS) inhibited phosphorylation of p38, JNK as well as mitogen-activated protein kinase (MAPK), and inhibitory kappa B-alpha (I kappa B alpha). This indicated that both compounds exert anti-inflammatory effects by inhibiting MAPKs and nuclear factor kappa B (NF-kappa B) pathways. (C) 2013 Elsevier Ltd. All rights reserved.
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