期刊
PHYTOCHEMISTRY
卷 74, 期 -, 页码 8-19出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2011.10.003
关键词
Cell wall; Cellulose; Galactomannan; Glucan; Glucomannan; Glucuronoarabinoxylan; Glucuronoxylan; Gums; Hemicellulose; Mannan; Matrix polysaccharides; Mixed-linked glucan; Xylan
Enzymes that make the polymer backbones of plant cell wall polysaccharides have proven to be recalcitrant to biochemical purification. Availability of mutational genetics and genomic tools paved the way for rapid progress in identifying genes encoding various cell wall glycan synthases. Mutational genetics, the primary tool used in unraveling cellulose biosynthesis, was ineffective in assigning function to any of the hemicellulosic, polymerizing glycan synthases. A combination of comparative genomics and functional expression in a heterologous system allowed identification of various cellulose synthase-like (Csl) sequences as being involved in the formation of beta-1,4-mannan, beta-1,4-glucan, and mixed-linked glucan. A number of xylose-deficient mutants have led to a variety of genes, none of which thus far possesses the motifs known to be conserved among polymerizing beta-glycan synthases. Except for xylan synthase, which appears to be an agglomerate of proteins just like cellulose synthase. Golgi glycan synthases already identified suggest that the catalytic polypeptide by itself is sufficient for enzyme activity, most likely as a homodimer. Several of the Csl genes remain to be assigned a function. The possibility of the involvement of various Csl genes in making more than one product remains. (C) 2011 Elsevier Ltd. All rights reserved.
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