期刊
PHYTOCHEMISTRY
卷 72, 期 10, 页码 1180-1191出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2010.12.008
关键词
2-D DIGE; Proteome; Salt-responsive proteins; Salt tolerance; Wheat leaf
资金
- National Key Natural Science Foundation of China [30830072]
- Chinese Ministry of Science and Technology [2009CB118300]
- National Key Project for Transgenic Crops [2008ZX08002-004, 2009ZX08002-017B]
Salt stress is a major abiotic stress that limits agricultural productivity in many regions of the world. To understand the molecular basis of the salt stress response in wheat (Triticum aestivum L), a proteomic approach was used to identify the salt stress-responsive proteins in an elite Chinese wheat cultivar. Zhengmai 9023, which exhibits a high yield, superior gluten quality and better biotic resistance. Three-week-old seedlings were treated with NaCl of four different concentrations (1.0%, 1.5%, 2.0%, and 2.5%). The total proteins from the leaves of untreated and NaCl-treated plants were extracted and separated by two-dimensional difference gel electrophoresis (2D-DIGE). A total of 2358 protein spots were detected on the gels, among which 125 spots showed a significant change in protein abundance, and 83 differentially expressed spots were localised on preparative gels. Using Q-TOF mass spectrometry, 52 salt-responsive spots were identified, which were classified into six functional categories that included transport-associated proteins, detoxifying enzymes, ATP synthase, carbon metabolism, protein folding, and proteins with unknown biological functions. Of the 52 differentially expressed proteins, 26 were up-regulated, 21 were down-regulated, and five spots showed multi-expression patterns. In particular, some important proteins for salt tolerance were found to be up-regulated in Zhengmai 9023 under salt stress, such as H+-ATPases, glutathione S-transferase, ferritin and triosephosphate isomerase. (C) 2010 Elsevier Ltd. All rights reserved.
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