期刊
PHYTOCHEMISTRY
卷 72, 期 4-5, 页码 423-429出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2011.01.001
关键词
Onobrychis viciifolia; Leguminosae; Sainfoin; Forage legume; Acylated flavonol glycosides; Cryoprobe NMR; MS/MS
资金
- European Commission [MRTN-CT-2006-035805]
- Medical Research Council [MC_U117533887] Funding Source: researchfish
- MRC [MC_U117533887] Funding Source: UKRI
Ten acylated flavonol glycosides were isolated from aqueous acetone extracts of the aerial parts of the forage legume, Onobrychis viciifolia, and their structures determined using spectroscopic methods. Among these were eight previously unreported examples which comprised either feruloylated or sinapoylated derivatives of 3-O-di- and 3-O-triglycosides of kaempferol (3,5,7,4'-tetrahydroxyflavone) or quercetin (3,5,7,3',4'-pentahydroxyflavone). The diglycosides were acylated at the primary Glc residue of O-alpha-Rhap(1 -> 6)-beta-Glcp (rutinose), whereas the triglycosides were acylated at the terminal Rha residues of the branched trisaccharides, O-alpha-Rhap(1 -> 2)[alpha-Rhap(1 -> 6)-beta-Galp or O-alpha-Rhap(1 -> 2)[alpha-Rhap(1 6)]-beta-Glcp. Identification of the primary 3 similar to O similar to linked hexose residues as either Gal or Glc was carried out by negative ion electrospray and serial MS, and cryoprobe NMR spectroscopy. Analysis of UV and MS spectra of the acylated flavonol glycosides provided additional diagnostic features relevant to direct characterisation of these compounds in hyphenated analyses. Quantitative analysis of the acylated flavonol glycosides present in different aerial parts of sainfoin revealed that the highest concentrations were in mature leaflets. (C) 2011 Elsevier Ltd. All rights reserved.
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