Determination of Nucleosides and Nucleobases in the Pollen of Typha Angustifolia by UPLC-PDA-MS

Introduction The pollen of Typha angustifolia L. has been used traditionally for the treatment of dysmenorrhea, stranguria and metrorrhagia in China. Recently, nucleosides and nucleobases have been proven as important bioactive compounds. Exploration of the nucleoside and nucleobase profiles from the pollen of T. angustifolia is important for improving its therapeutic value and could be convenient for its quality evaluation. Objective To establish an UPLC-PDA-MS method for simultaneous determination of nucleosides and nucleobases in the pollen of T. angustifolia. Methodology The analysis was performed on an Acuity UPLCHSS T3 column with a gradient elution of 5?mm ammonium acetate and methanol solution at a flow rate of 0.3?mL/min. Results Satisfactory separation of these compounds was obtained in less than 12?min. All calibration curves showed good linear regression (r2?>?0.9995). The method provided good accuracy, precision, recovery, and sensitivity for the quantification of the 10 compounds analysed. Conclusion The UPLC method established is very helpful for optimising their content and could be convenient for quality evaluation of the pollen of T. angustifolia, which has not been reported as far as we are aware. Copyright (C) 2011 John Wiley & Sons, Ltd.