期刊
PHYSIOLOGICAL GENOMICS
卷 46, 期 5, 页码 177-190出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/physiolgenomics.00191.2013
关键词
transcriptome; salinity stress; pathways; Chinese mitten crab; Eriocheir sinensis
资金
- Special Fund for Agroscientific Research in the Public Interest [201003020, 201203065]
- National Twelfth Five-Year Plan for Science Technology [2012BAD25B03]
- National Basic Research Program (973Program) [2009CB118702]
- Shanghai Committee of Science and Technology, China [10JC1404100]
- National Natural Science Foundation of China [31172422, 31001098]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20100076120006]
- Shanghai University Knowledge Service Platform Shanghai Ocean University aquatic animal breeding center [ZF1206]
- E-Institute of Shanghai Municipal Education Commission [E03009]
- East China Normal University innovation fund
A total of 276.9 million reads were obtained and assembled into 206, 371 contigs with an average length of 614 bp and N50 of 1,470 bp. Comparison of digital gene expression between treatment and control group reveals 1,151 and 941 genes were significantly differentially expressed in crab gill and muscle, respectively. In gill and muscle, protein ubiquitination, ubiquinone biosynthesis, oxidative phosphorylation, and mitochondria dysfunction pathways were the top pathways differentially expressed following the challenge. EIF 2 signaling pathway and IGF-1 signaling pathway were the top ones among the signal-related pathways. Most of the amino acid metabolism pathways were found to be involved in this process. The expression patterns of 15 differentially expressed genes were validated by quantitative real-time RT-PCR (average correlation coefficient 0.80). This is the first report of expression analysis of genes and pathways involved in osmoregulation of Eriocheir sinensis through transcriptome sequencing. The findings of this study will further promote the understanding of the underlying molecular mechanism of salinity stress adaptation for crustacean species.
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