期刊
PHYSIOLOGICAL GENOMICS
卷 43, 期 8, 页码 417-437出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/physiolgenomics.00249.2010
关键词
pulmonary arterial hypertension; estrogen; CCAAT enhancer binding protein; cytochrome P450 1B1; c-FOS
资金
- Biotechnology and Biological Sciences Research Council (BBSRC)
- British Pharmacological Society
- Scottish Funding Council
- Medical Research Council
- Knowledge Transfer Network
- MRC [G0801171] Funding Source: UKRI
- Medical Research Council [G0801171] Funding Source: researchfish
White K, Loughlin L, Maqbool Z, Nilsen M, McClure J, Dempsie Y, Baker AH, MacLean MR. Serotonin transporter, sex, and hypoxia: microarray analysis in the pulmonary arteries of mice identifies genes with relevance to human PAH. Physiol Genomics 43: 417-437, 2011. First published February 8, 2011; doi:10.1152/physiolgenomics.00249.2010.-Pulmonary arterial hypertension (PAH) is up to threefold more prevalent in women than men. Female mice overexpressing the serotonin transporter (SERT; SERT+ mice) exhibit PAH and exaggerated hypoxia-induced PAH, whereas male SERT+ mice remain unaffected. To further investigate these sex differences, microarray analysis was performed in the pulmonary arteries of normoxic and chronically hypoxic female and male SERT+ mice. Quantitative RT-PCR analysis was employed for validation of the microarray data. In relevant groups, immunoblotting was performed for genes of interest (CEBP beta, CYP1B1, and FOS). To translate clinical relevance to our findings, CEBP beta, CYP1B1, and FOS mRNA and protein expression was assessed in pulmonary artery smooth muscle cells (PASMCs) derived from idiopathic PAH (IPAH) patients and controls. In female SERT+ mice, multiple pathways with relevance to PAH were altered. This was also observed in chronically hypoxic female SERT+ mice. We selected 10 genes of interest for qRT-PCR analysis (FOS, CEBP beta, CYP1B1, MYL3, HAMP2, LTF, PLN, NPPA, UCP1, and C1S), and 100% concordance was reported. Protein expression of three selected genes, CEBP beta, CYP1B1, FOS, was also upregulated in female SERT+ mice. Serotonin and 17 beta-estradiol increased CEBP beta, CYP1B1, and FOS protein expression in PASMCs. In addition, CEBP beta, CYP1B1, and FOS mRNA and protein expression was also increased in PASMCs derived from IPAH patients. Here, we have identified a number of genes that may predispose female SERT+ mice to PAH, and these findings may also be relevant to human PAH.
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