期刊
PHYSIOLOGICAL GENOMICS
卷 35, 期 3, 页码 283-295出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/physiolgenomics.90213.2008
关键词
microarray analysis; metabolic signaling; quantitative real-time polymerase chain reaction; neuropeptide
资金
- National Science Foundation
- Idaho Experimental Program to Stimulate Competitive Research (EPSCoR) [EPS0447689]
- National Institutes of Health [P20-RR016448]
- National Center for Research Resources
Drew RE, Rodnick KJ, Settles M, Wacyk J, Churchill E, Powell MS, Hardy RW, Murdoch GK, Hill RA, Robison BD. Effect of starvation on transcriptomes of brain and liver in adult female zebrafish (Danio rerio). Physiol Genomics 35: 283-295, 2008. First published August 26, 2008; doi: 10.1152/physiolgenomics.90213.2008.-We used microarray and quantitative real-time PCR (qRT-PCR) analyses in adult female zebrafish (Danio rerio) to identify metabolic pathways regulated by starvation in the liver and brain. The transcriptome of whole zebrafish brain showed little response to 21 days of starvation. Only agouti-related protein 1 (agrp1) significantly responded, with increased expression in brains of starved fish. In contrast, a 21-day period of starvation significantly downregulated 466 and upregulated 108 transcripts in the liver, indicating an overall decrease in metabolic activity, reduced lipid metabolism, protein biosynthesis, proteolysis, and cellular respiration, and increased gluconeogenesis. Starvation also regulated expression of many components of the unfolded protein response, the first such report in a species other than yeast (Saccharomyces cerevisiae) and mice (Mus musculus). The response of the zebrafish hepatic transcriptome to starvation was strikingly similar to that of rainbow trout (Oncorhynchus mykiss) and less similar to mouse, while the response of common carp (Cyprinus carpio) differed considerably from the other three species.
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