Mapping of quantitative trait loci for cholesterol, LDL, HDL, and triglyceride serum concentrations in pigs

Gallardo D, Pena RN, Amills M, Varona L, Ramirez O, Reixach J, Diaz I, Tibau J, Soler J, Prat-Cuffi JM, Noguera JL, Quintanilla R. Mapping of quantitative trait loci for cholesterol, LDL, HDL and triglyceride serum concentrations in pigs. Physiol Genomics 35: 199-209, 2008. First published September 23, 2008; doi: 10.1152/physiolgenomics.90249.2008.-The fine mapping of polymorphisms influencing cholesterol (CT), triglyceride (TG), and lipoprotein serum levels in human and mouse has provided a wealth of knowledge about the complex genetic architecture of these traits. The extension of these genetic analyses to pigs would be of utmost importance since they constitute a valuable biological and clinical model for the study of coronary artery disease and myocardial infarction. In the present work, we performed a whole genome scan for serum lipid traits in a half-sib Duroc pig population of 350 individuals. Phenotypic registers included total CT, TG, and low (LDL)- and high (HDL)-density lipoprotein serum concentrations at 45 and 190 days of age. This approach allowed us to identify two genomewide significant quantitative trait loci (QTL) for HDL-to-LDL ratio at 45 days (SSC6, 84 cM) and for TG at 190 days (SSC4, 23 cM) as well as a number of chromosomewide significant QTL. The comparison of QTL locations at 45 and 190 days revealed a notable lack of concordance at these two time points, suggesting that the effects of these QTL are age specific. Moreover, we have observed a considerable level of correspondence among the locations of the most significant porcine lipid QTL and those identified in humans. This finding might suggest that, in mammals, diverse polymorphisms located in a common set of genes are involved in the genetic variation of serum lipid levels.