期刊
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
卷 86, 期 -, 页码 43-48出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.pmpp.2014.03.001
关键词
Chinese jujube; jujube witches' broom disease; Phytoplasma; Suppression subtractive hybridization
资金
- National Science and Technology Support Plan of China [2013BAD14B03]
- Natural Science Foundation of Hebei Province [C2010000679, C2009000534]
- Provincial Key Technology R&D Program of Hebei, China [11230606D-6]
Jujube witches' broom (JWE) is a destructive disease for Chinese jujube caused by phytoplasma. A suppression subtractive hybridization library of resistant cultivar 'Xingguang' was constructed under phytoplasma stress to identify genes related to JWB resistance. 77 of 200 unique expressed sequence tags had significant sequence homologies and were classified into 10 functional groups. The most abundant group was disease/defense (20.8%), which was consistent with the phytoplasma stress. These differentially expressed genes provide the groundwork for addressing the plant-phytoplasma interaction. Meanwhile, the expression of five selected genes (TLP, PR10, HSP70, ERF, kinase-related protein) was confirmed to upregulate at different infection periods. (C) 2014 Elsevier Ltd. All rights reserved.
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