期刊
PHYSIOLOGIA PLANTARUM
卷 153, 期 3, 页码 355-364出版社
WILEY-BLACKWELL
DOI: 10.1111/ppl.12249
关键词
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资金
- National Basic Research Program of China [2014CB1 38701]
- Natural Science Foundation of China [30830081]
A full length cDNA encoding a myo-inositol transporter-like protein, named as MfINT-like, was cloned from Medicago sativa subsp. falcata (herein falcata), a species with greater cold tolerance than alfalfa (M. sativa subsp. sativa). MfINT-like is located on plasma membranes. MfINT-like transcript was induced 2-4h after exogenous myo-inositol treatment, 24-96h with cold, and 96h by salinity. Given that myo-inositol accumulates higher in falcata after 24h of cold treatment, myo-inositol is proposed to be involved in cold-induced expression of MfINT-like. Higher levels of myo-inositol was observed in leaves of transgenic tobacco plants overexpressing MfINT-like than the wild-type but not in the roots of plants grown on myo-inositol containing medium, suggesting that transgenic plants had higher myo-inositol transport activity than the wild-type. Transgenic plants survived better to freezing temperature, and had lower ion leakage and higher maximal photochemical efficiency of photosystem II (F-v/F-m) after chilling treatment. In addition, greater plant fresh weight was observed in transgenic plants as compared with the wild-type when plants were grown under drought or salinity stress. The results suggest that MfINT-like mediated transport of myo-inositol is associated with plant tolerance to abiotic stresses.
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