期刊
PHYSIOLOGIA PLANTARUM
卷 143, 期 4, 页码 309-315出版社
WILEY
DOI: 10.1111/j.1399-3054.2011.01506.x
关键词
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资金
- China National '863' High-Tech program [2011AA100605]
- Natural Science Foundation of Chongqing Science & Technology Commission [CSTC2009 BB5309]
- Fundamental Research Funds for the Central Universities [XDJK2010C087]
- Fund for Excellent Young Scientists of Universities in Chongqing City
Atropa belladonna is officially deemed as the commercial plant to produce scopolamine in China. In this study we report the simultaneous overexpression of two functional genes involved in biosynthesis of scopolamine, which encode the upstream key enzyme putrescine N-methyltransferase (PMT) and the downstream key enzyme hyoscyamine 6 beta-hydroxylase (H6H), respectively, in transgenic herbal plants Atropa belladonna. Analysis of gene expression profile indicated that both pmt and h6h were expressed at a higher level in transgenic lines, which would be favorable for biosynthesis of scopolamine. High-performance liquid chromatography result suggested that transgenic lines could produce higher accumulation of scopolamine at different levels compared with wild-type lines. Scopolamine content increased to 7.3-fold in transgenic line D9 compared with control lines. This study not only confirms that co-overexpression of pmt and h6h is an ideal method to improve the biosynthetic capacity of scopolamine but also successfully cultivates the transgenic line D9, which significantly enhanced the scopolamine accumulation. Our research can serve as an alternative choice to provide scopolamine resources for relative industry, which is more competitive than conventional market.
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