期刊
WATER RESEARCH
卷 76, 期 -, 页码 43-52出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2015.02.042
关键词
Denitrification; Functional genes; Hexavalent chromium; High-throughput sequencing; Microbial community
资金
- Major Science and Technology Program of China for Water Pollution Control and Treatment [2012ZX07101-002-004, 2012ZX07506-004-004]
- National Natural Science Foundation of China [51378252]
- Jiangsu Science and Technology Support Program of China [BE2013704]
- Environmental Protection Research Foundation of Jiangsu Province (China) [2012045]
In this study, a lab-scale expanded granular sludge bed reactor was continuously operated to treat high-nitrate wastewater containing different concentrations of hexavalent chromium (Cr(VI)). Nearly complete nitrate removal was achieved even at 120 mg/L influent Cr(VI). Pyrosequencing of 16S rRNA gene showed that Cr(VI) decreased the biodiversity of the bacterial community and potential denitrifiers. Proteobacteria dominated in the bioreactor, and Betaproteobacteria had increased abundance after Cr(VI) feeding. Thauera and Halomonas were the two predominant genera in the bioreactor fed with Cr(VI), demonstrating opposite responses to the Cr(VI) stress. Metagenomic analysis indicated that Cr(VI) feeding posed no obvious effect on the overall function of the bacterial community, but altered the abundance of specific denitrifying genes, which was evidenced by quantitative real time PCR. This study revealed that Halomonas mainly contributed to the denitrification under no or low Cr(VI) stress, while Thauera played a more important role under high Cr(VI) stress. (C) 2015 Elsevier Ltd. All rights reserved.
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