期刊
WATER RESEARCH
卷 69, 期 -, 页码 90-99出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2014.11.010
关键词
Ammonia inhibition; Acetotrophic methanogens; Acetate-utilizing bacteria; Metabolic pathway dynamics; Pyrosequencing; Stable isotope signature
资金
- DRRT Ile de France in the framework of the CPER-LABE
- Collaborative Innovation Center for Regional Environmental Quality
- National Natural Science Foundation of China [21177096, 51378375, 51178327]
- China 111 Project
Ammonia inhibition represents a major operational issue for anaerobic digestion. In order to refine our understanding of the terminal catabolic steps in thermophilic anaerobic digestion under ammonia stress, we studied batch thermophilic acetate fed experiments at low (0.26 g L-1) and high (7.00 g L-1) Total Ammonia Nitrogen concentrations (TAN). Although methane production started immediately for all incubations and resulted in methane yields close to stoichiometric expectations, a 62-72% decrease of methanogenic rate was observed throughout the incubation at 7.00 g L-1 of TAN compared to 0.26 g L-1. Stable Isotope Probing analysis of active microbial communities in C-13-acetate fed experiments coupled to automated ribosomal intergenic spacer analysis and 16S rDNA pyrotag sequencing confirmed that microbial communities were similar for both TAN conditions. At both TAN levels, the C-13-labeled bacterial community was mainly affiliated to Clostridia-relatives, with OPB54 bacteria being the most abundant sequence in the heavy DNA 16S rDNA pyrotag library. Sequences closely related to Methanosarcina thermophila were also abundantly retrieved in the heavy DNA fractions, showing that this methanogen was still actively assimilating labeled carbon from acetate at free ammonia nitrogen concentrations up to 916 mg L-1. Stable isotopic signature analysis of biogas, measured in unlabeled
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