期刊
WATER RESEARCH
卷 75, 期 -, 页码 123-130出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2015.02.035
关键词
Resazurin; Biosensor; Toxicity; Anaerobic digestion; Pentachlorophenol
资金
- Public Utility Board (PUB), Singapore
- Nanyang Environmental and Water Research Institute (NEWRI), Nanyang Technical University (NTU), Singapore [PUB IDD 21100/36/6]
A rapid fluorescence measurement based on resazurin reduction was developed and applied for the detection of toxicants/inhibitors to anaerobic digestion metabolism. By initially using a pure facultative anaerobic strain, Enterococcus faecalis as a model organism, this technique proved to be fast and sensitive when detecting the model toxicant, pentachlorophenol (PCP). The technique revealed significant metabolic changes in Enterococcus faecalis with a PCP spike ranging from 0.05 to 100 mg/L, and could detect PCP's toxicity to E. faecalis at a concentration of only 0.05 mg/L in 8 min. Furthermore, by extending this technique to a mixed anaerobic sludge, not only could the effect of 0.05-100 mg/L PCP be determined on anaerobic digestion metabolism within 10 min, but also its rate of biogas production. These results suggest that a resazurin-based fluorescence measurement can potentially be incorporated into a microfluidic system to develop a biosensor for the real-time monitoring, control and early warning of toxicant/inhibitor loads in the influent to an anaerobic digestion system. (C) 2015 Elsevier Ltd. All rights reserved.
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