期刊
VIRUS RESEARCH
卷 197, 期 -, 页码 108-115出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2014.12.024
关键词
Porcine coronaviruses; Porcine APN; Pathogenesis; Porcine epidemic diarrhea virus; Transgenic mouse model
类别
资金
- Animal and Plant Quarantine Agency of Korea [Z-AD-14-2009-10-02]
- Technology Development Program for Agriculture and Forestry
- Korean Research Foundation [20120008358, 2011-0023942, 211-2006-2-E00027]
- Ministry for Food, Agriculture, Forestry and Fisheries
- National Research Foundation of Korea [2011-0023942] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Porcine coronavirus infections have known as they are specific to pigs with predominantly enteric or respiratory diseases. No laboratory animal model is yet been developed in porcine coronaviruses study. Here, we report that development of a transgenic mouse model expressing porcine APN which is susceptible to porcine coronavirus infection. The porcine APN transgene was constructed by fusing with mouse proximal APN promoter at 5' terminus and bovine growth hormone polyadenylation site at its 3' terminus. After screen on pubs from the microinjected mice, we confirmed two transgenic lines expressing porcine APN in various organs. We confirmed the susceptibility to porcine epidemic diarrhea virus, one of the porcine coronaviruses. These transgenic mice will be an important tool for research into the porcine coronaviruses. (C) 2014 Elsevier B.V. All rights reserved.
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