期刊
VIRUS GENES
卷 51, 期 1, 页码 122-131出版社
SPRINGER
DOI: 10.1007/s11262-015-1223-8
关键词
ETA-converting bacteriophages; Staphylococcus aureus; Complete genome sequences; Virion protein patterns
资金
- Internal Grant Agency of the Ministry of Health of the Czech Republic [NT12395-5]
- Czech Ministry of Education, Youth and Sports [AdmireVet CZ.1.05/2.1.00/01.0006-ED0006/01/01]
- CEITEC (Central European Institute of Technology) Proteomics Core Facility - European Regional Development Fund [CZ.1.05/1.1.00/02.0068]
- Czech Science Foundation [P206-12-G151]
Exfoliative toxin A (ETA)-coding temperate bacteriophages are leading contributors to the toxic phenotype of impetigo strains of Staphylococcus aureus. Two distinct eta gene-positive bacteriophages isolated from S. aureus strains which recently caused massive outbreaks of pemphigus neonatorum in Czech maternity hospitals were characterized. The phages, designated phi B166 and phi B236, were able to transfer the eta gene into a prophageless S. aureus strain which afterwards converted into an ETA producer. Complete phage genome sequences were determined, and a comparative analysis of five designed genomic regions revealed major variances between them. They differed in the genome size, number of open reading frames, genome architecture, and virion protein patterns. Their high mutual sequence similarity was detected only in the terminal regions of the genome. When compared with the so far described eta phage genomes, noticeable differences were found. Thus, both phages represent two new lineages of as yet not characterized bacteriophages of the Siphoviridae family having impact on pathogenicity of impetigo strains of S. aureus.
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