期刊
VIROLOGY
卷 485, 期 -, 页码 322-329出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2015.08.009
关键词
Flavivirus; Dengue virus; Kunjin virus; RNA interference; SfRNA; Mosquito; Arbovirus
类别
资金
- NIAID award through the Rocky Mountain Regional Center of Excellence [U54 AI-065357]
- USDA NIFA NNF training grant [2010-38420-20367]
- National Institute of Allergy and Infectious Disease, National Institutes of Health [AI067380]
- NIFA [581234, 2010-38420-20367] Funding Source: Federal RePORTER
Productive arbovirus infections require mechanisms to suppress or circumvent the cellular RNA interference (RNA pathway, a major antiviral response in mosquitoes. In this study, we demonstrate that two flaviviruses, Dengue virus and Kunjin virus, significantly repress siRNA-mediated RNAi in infected human cells as well as during infection of the mosquito vector Culex quinquefasciatus. Arthropod-borne flaviviruses generate a small structured non-coding RNA from the viral 3' UTR referred to as sfRNA. Analysis of infections with a mutant Kunjin virus that is unable to generate appreciable amounts of the major sfRNA species indicated that RNAi suppression was associated with the generation of the non-coding sfRNA. Co-immunoprecipitation of sfRNA with RNAi mediators Dicer and Ago2 suggest a model for RNAi suppression. Collectively, these data help to establish a clear role for sfRNA in RNAi suppression and adds to the emerging impact of viral long non-coding RNAs in modulating aspects of anti-viral immune processes. (C) 2015 Elsevier Inc. All rights reserved.
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