期刊
PHYSICAL REVIEW LETTERS
卷 113, 期 13, 页码 -出版社
AMER PHYSICAL SOC
DOI: 10.1103/PhysRevLett.113.133902
关键词
-
资金
- National Institutes of Health, National Institute of General Medical Sciences [R01GM085437]
To extract from an image of a single nanoscale object maximum physical information about its position, we propose and demonstrate a framework for pupil-plane modulation for 3D imaging applications requiring precise localization, including single-particle tracking and superresolution microscopy. The method is based on maximizing the information content of the system, by formulating and solving the appropriate optimization problem-finding the pupil-plane phase pattern that would yield a point spread function (PSF) with optimal Fisher information properties. We use our method to generate and experimentally demonstrate two example PSFs: one optimized for 3D localization precision over a 3 mu m depth of field, and another with an unprecedented 5 mu m depth of field, both designed to perform under physically common conditions of high background signals.
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