期刊
VIROLOGY
卷 485, 期 -, 页码 460-466出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2015.08.017
关键词
HPV16; Hypermutation; APOBEC3; Cervical cancer; Next-Generation Sequencing
类别
资金
- Japan Society for the Promotion of Science [26861318]
- Founding Program for Next Generation World-Leading Researchers [LS051]
- YASUDA Medical Foundation
- Takeda Science Foundation
- Association for Research on Lactic Acid Bacteria
- Grants-in-Aid for Scientific Research [25670713, 24689064, 26861318, 26293358, 26460993] Funding Source: KAKEN
Human papillomavirus type 16(HPV16) is a major cause of cervical cancer. We previously demonstrated that C-to-T and G-to-A hypermutations accumulated in the HPV16 genome by APOBEC3 expression in vitro. To investigate in vivo characteristics of hypermutation, differential DNA denaturation-PCR (3D-PCR) was performed using three clinical specimens obtained from HPV16-positive cervical dysplasia, and detected hypermutation from two out of three specimens. One sample accumulating hypermutations in both E2 and the long control region (LCR) was further subjected to Next-Generation Sequencing, revealing that hypermutations spread across the LCR and all early genes. Notably, hypermutation was more frequently observed in the LCR, which contains a viral replication origin and the early promoter. APOBEC3 expressed abundantly in an HPV16-positive cervix, suggesting that single-stranded DNA exposed during viral replication and transcription may be efficient targets for deamination. The results further strengthen a role of APOBEC3 in introducing HPV16 hypermutation in vivo. (C) 2015 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据