期刊
VIROLOGY
卷 483, 期 -, 页码 44-53出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2015.04.007
关键词
EBV; BGLF3.5; Lytic replication; uORF; Termination/re-initiation
类别
资金
- Ministry of Education, Culture, Sports, Science and Technology [30470165, 15K08494, 25293109]
- Ministry of Health, Labor and Welfare [H26-Nanchi-013]
- Uehara Memorial Research Fund
- Takeda Science Foundation
- Kanae Foundation for Promotion of Medical Science
- Kitamura Memorial Research Fund
- General Assembly of the Japanse Association of Medical Sciences
- Grants-in-Aid for Scientific Research [25293109, 15K08494] Funding Source: KAKEN
The Epstein-Barr virus (EBV) predominantly establishes a latent infection in B lymphocytes, but a small percentage of infected cells switch from the latent state to the lytic cycle, leading to potent viral DNA replication and progeny viruses production. We here focused on a lytic gene BGLF3.5, and first established BGLF3.5 mutants by marker cassette insertion. Unexpectedly, this insertion mutant failed to produce BGLF4 protein and thus progeny production was severely inhibited. Then we carefully made two point mutant viruses (stop codon insertion or frame-shift mutation) and found that BGLF3.5 is not essential for EBV lytic replication processes, such as viral gene expression, DNA replication, or progeny production in the HEK293 cells although its homolog in murine gammaherpesvirus 68 (MHV-68) was reported to be essential. In addition, we examined the roles of two short, upstream open reading frames within the 5'UTR of BGLF3.5 gene in translation of BGLF4. (C) 2015 Elsevier Inc. All rights reserved.
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