期刊
VIROLOGY
卷 486, 期 -, 页码 54-62出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2015.08.032
关键词
Plant virus; RNA virus; Cap-independent translation; RNA structure; IRES; Readthrough; Leaky scanning; Tombusviridae; Necrovirus; Catmovirus
类别
资金
- NSERC
Tobacco necrosis virus (TNV-D) has a plus-strand RNA genome that is neither 5' capped nor 3' polyadenylated. Instead, it utilizes a 3' cap-independent translational enhancer (3'CITE) located in its 3' untranslated region (UTR) for translation of its proteins. We have examined the protein expression strategies used by TNV-D and our results indicate that: (i) a base pairing interaction between conserved ACCA and UGGU motifs in the genomic 5'UTR and 3'CITE, respectively, is not required for efficient plant cell infection, (ii) similar potential 5'UTR-3'CITE interactions in the two viral subgenomic mRNAs are not needed for efficient translation of viral proteins in vitro, (iii) a small amount of capsid protein is translated from the viral genome by a largely 3'CITE-independent mechanism, (iv) the larger of two possible forms of capsid protein is efficiently translated, and (v) p7b is translated from subgenomic mRNA1 by a leaky scanning mechanism. (C) 2015 Elsevier Inc. All rights reserved.
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