期刊
VIROLOGY
卷 478, 期 -, 页码 75-85出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2015.02.005
关键词
Coronavirus; Envelope protein; Protein transport/localization; Virus assembly; Live-cell imaging; FRAP; CLEM
类别
资金
- National Institutes of Health [AI53704]
- Protein Structure Initiative (PSI) [GM094599]
- American Society for Microbiology
- Science Foundation Arizona Graduate Research Fellowship
Coronaviruses (CoVs) assemble at endoplasmic reticulum Golgi intermediate compartment (ERGIC) membranes and egress from cells in cargo vesicles. Only a few molecules of the envelope (E) protein are assembled into virions. The role of E in morphogenesis is not fully understood. The cellular localization and dynamics of mouse hepatitis Coy A59 (MHV) E protein were investigated to further understanding of its role during infection. E protein localized in the ERGIC and Golgi with the amino and carboxy termini in the lumen and cytoplasm, respectively. E protein does not traffic to the cell surface. MHV was genetically engineered with a tetracysteine tag at the carboxy end of E. Fluorescence recovery after photobleaching (FRAP) showed that E is mobile in ERGIC/Golgi membranes. Correlative light electron microscopy (CLEM) confirmed the presence of E in Golgi cisternae. The results provide strong support that E proteins carry out their function(s) at the site of budding/assembly. (C) 2015 Elsevier Inc. All rights reserved.
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