期刊
VIROLOGY
卷 478, 期 -, 页码 39-49出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2015.01.031
关键词
Tospoviruses; NSm dimer; N dimer; NSm-N interaction; BiFC; Movement protein topology; Mixed infections
类别
资金
- Spanish granting agency DGICYT [BIO2011-25018]
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) [564633/2010-0]
- CAPES (Conselho de Aperfeicoamento de Pessoal de Nivel Superior) [564633/2010-0]
- FAP-DF (Fundacao de Apoio a Pesquisa do Distrito Federal) [2009/00102-2]
Tospovirus is the only genus containing virus species which infect plants in the Bunyaviridae family. The aims of this study were to understand the in vivo membrane association of the movement protein (NSm) of the tospovirus species Bean necrotic mosaic virus, Chrysanthemum stem necrosis virus, Tomato chlorotic spot virus and Tomato spotted wilt virus and the homologous and heterologous interactions among NSm and nucleocapsid protein (N). The results obtained by bimolecular fluorescence complementation (BiFC) assay and chemical treatments after membrane fractionation revealed that the four NSm proteins are associated with the biological membranes with the N- and C-termini oriented to the cytoplasm. BiFC analysis for protein protein interactions showed: i) dimer formation for all NSm and N proteins; ii) interaction between NSm and the cognate N and iii) heterologous interactions between the NSm and N proteins. The implications of these interactions in the life cycle of tospoviruses are discussed. (C) 2015 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据