4.4 Article

Expression and partial characterisation of rabbit haemorrhagic disease virus non-structural proteins

期刊

VIROLOGY
卷 484, 期 -, 页码 69-79

出版社

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2015.05.004

关键词

Calicivirus; Replication; Rabbit haemorrhagic disease virus; RNA virus replication; Non-structural proteins; Calicivirus polymerase; Polyprotein processing; Golgi; Oligomerisation

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资金

  1. Invasive Animals Cooperative Research Centre (IA CRC) [IACRC 3.L.4]
  2. IA CRC
  3. University of Canberra

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The intracellular replication and molecular virulence mechanisms of Rabbit haemorrhagic disease virus (RHDV) are poorly understood, mainly due to the lack of an effective cell culture system for this virus. To increase our understanding of RHDV molecular biology, the subcellular localisation of recombinant nonstructural RHDV proteins was investigated in transiently transfected rabbit kidney (RK-13) cells. We provide evidence for oligomerisation of p23, and an ability of the viral protease to cleave the p16:p23 junction in trans, outside the context of the nascent polyprotein chain. Notably, expression of the viral polymerase alone and in the context of the entire RHDV polyprotein resulted in a redistribution of the Golgi network. This suggests that, similar to other positive-strand RNA viruses, RHDV may recruit membranes of the secretory pathway during replication, and that the viral polymerase may play a critical role during this process. (C) 2015 Elsevier Inc. All rights reserved.

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