期刊
VETERINARY MICROBIOLOGY
卷 180, 期 1-2, 页码 136-141出版社
ELSEVIER
DOI: 10.1016/j.vetmic.2015.07.030
关键词
Q Fever; Coxiella burnetii; Reproductive disorders; Zoonosis; MLVA-6 typing; Wild carnivores
资金
- Fundacao para a Ciencia e a Tecnologia (FCT) [PTDC/SAU/SAP/115266/2009]
- FCT [SFRH/BPD/35842/2007, PTDC/CVT/117794/2010, PEst-C/MAR/LA0017/2013]
- Fundo Social Europeu (III Quadro Comunitario de Apoio)
- European Funds through COMPETE
- Fundação para a Ciência e a Tecnologia [PTDC/SAU-SAP/115266/2009, SFRH/BPD/35842/2007, PTDC/CVT/117794/2010] Funding Source: FCT
Coxiella burnetii is the etiological agent of Q fever or Coxiellosis, a zoonosis mainly affecting domestic ruminants. Information on the population structure and epidemiology of C bumetii in animals is scarce in Portugal. Evidence of C bumetti infection was sought in domestic, wild and captive animals based on the detection of bacterial DNA. Tissue samples from 152 domestic animals (cattle = 24, goats = 51, sheep = 76 and swine = 1), 55 wild carnivores (Egyptian mongoose = 45, red fox = 4, common genet=3, weasel = 2 and European badger = 1) and 22 zoo animals (antelopes = 15, impala = 1; rhinoceros = 1, deer = 2, zebras =2 and giraffe = 1) were screened by nested-touchdown PCR. Cloacae swabs from 19 griffon vultures were also analysed. Among the domestic ruminants, goats presented the highest prevalence of infection (23.53%), followed by cattle, (20.83%) and sheep (10.53%). C bumetii DNA was also detected in five Egyptian mongooses and two antelopes and one giraffe. Using a 6-locus multiple-locus variable-number tandem repeat analysis (MLVA-6) six complete genotypes, T, I and CM and the first reported CN, CO and CP, were identified, respectively, in small ruminants and Egyptian mongooses. Clustering analysis of genotypes exposed four distinct groups, according to detection source, enlightening an apparent association between C bumetii genotype and host. (C) 2015 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据