期刊
VETERINARY JOURNAL
卷 206, 期 1, 页码 115-117出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.tvjl.2015.06.014
关键词
Calicivirus; Norovirus; Cat; Sequence analysis; Real-time PCR
资金
- Carlos Chagas Filho Foundation for Research Support of the State of Rio de Janeiro [E-26/110.909/2013]
- Brazilian National Council for Scientific and Technological Development [023/2011]
- Fluminense Federal University Office of Research and Graduate Studies
- Oswaldo Cruz Institute
Feline caliciviruses (FCVs) have occasionally been described in cats in association with enteric disease, but an etiological role for these viruses in acute gastroenteritis is still unclear. In this study, molecular characterization of FCV and feline norovirus (FNoV) was undertaken using real-time PCR (RT-PCR) and sequence analysis of the ORF1 region in fecal specimens from 29 diarrheic cats. The specimens were also screened for parvovirus, coronavirus, astrovirus and group A rotavirus. A quantitative one step RT-PCR was also performed to detect and quantitate NoV genogroup IV and the role of these animal caliciviruses in feline gastroenteritis was investigated. This is the first description of enteric FCV and FNoV in South America. (C) 2015 Elsevier Ltd. All rights reserved.
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