期刊
VETERINARY JOURNAL
卷 205, 期 1, 页码 107-109出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.tvjl.2015.02.007
关键词
Classical swine fever virus; E2; Glycosylation; Mammalian expression; Medium-direct-coating ELISA
资金
- Ministry of Science and Technology, Taiwan [MOST 103-2325-B-037-007, NSC 102-2320-B-038-043-MY2]
- Ministry of Health and Welfare, Taiwan [MOHW103-TD-B-111-05]
- National Health Research Institutes, Taiwan [NHRI-EX103-10238SC]
- 103NSYSU-KMU Joint Research Project [NSYSUKMU103 I-003]
- Grant of Biosignature in Colorectal Cancers, Academia Sinica, Taiwan
- Kaohsiung Medical University, Taiwan [KMU-TP103C00, KMU-DT103005]
The envelope glycoprotein E2 of classical swine fever virus (CSFV) is widely used as a marker for measuring vaccine efficacy and antibody titer. The glycosylation profile of E2 may affect the immunogenicity of the vaccine and the timing of re-vaccination. In this study, a human embryonic kidney cell line was used to secrete fully-glycosylated CSFV E2, which was then coated onto ELISA plates without purification or adjustment. The resulting E2-secreting medium-direct-coating (E2-mDc) ELISA was successfully used to measure anti-E2 antibody titers in vaccinated and field pig sera samples. Compared with a virus neutralization test (as standard), the E2-mDc ELISA was found to be more accurate (90%) than a commercial CSFV antibody diagnostic kit (62%). In conclusion, the mammalian cell-secreted antigen can provide cheap, accurate and effective assays for vaccine efficacy and disease diagnoses. (C) 2015 Elsevier Ltd. All rights reserved.
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