期刊
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
卷 164, 期 3-4, 页码 170-178出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetimm.2015.02.006
关键词
In ovo; Toll-like receptor 2; Lipoteichoic acid; Infectious laryngotracheitis virus; Lung
资金
- University of Calgary Faculty of Veterinary Medicine
- Canadian Poultry Research Council [AMN048]
- Alberta Livestock and Meat Agency [2011R037R]
- Natural Sciences and Engineering Research Council of Canada through a NSERC-RTI grant [421963-2012]
Toll-like receptor (TLR) ligands are pathogen associated molecular patterns (PAMPs) recognized by the TLRs resulting in induction of host innate immune responses. One of the PAMPs that binds to TLR2 and cluster of differentiation (CD) 14 is lipotechoic acid (LTA), which activates downstream signals culminating in the release of pro-inflammatory cytokines. In this study, we investigated whether in ovo LTA delivery leads to the induction of antiviral responses against post-hatch infectious laryngotracheitis virus (ILTV) infection. We first delivered the LTA into embryo day (ED)18 eggs via in ovo route so that the compound is available at the respiratory mucosa. Then the LTA treated and control ED18 eggs were allowed to hatch and the hatched chicken was infected with ILTV intratracheally on the day of hatch. We found that in ovo delivered LTA reduces ILTV infection post-hatch. We also found that in ovo delivery of LTA significantly increases mRNA expression of pro-inflammatory mediators in pre-hatch embryo lungs as well as mononuclear cell infiltration, predominantly macrophages, in lung of post-hatch chickens. Altogether, the data suggest that in ovo delivered LTA could be used to reduce ILTV infection in newly hatched chickens. (C) 2015 Elsevier B.V. All rights reserved.
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