期刊
VACCINE
卷 33, 期 20, 页码 2328-2334出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2015.03.043
关键词
Bacillus subtilis; Spores; Adjuvants; DNA vaccine; HPV-16
资金
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior CAPES
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo-FAPESP [2011/13805-1]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [11/13805-1] Funding Source: FAPESP
Recently, Bacillus subtilis spores were shown to be endowed with strong adjuvant capacity when co-administered with purified antigenic proteins. In the present study we assessed whether spores possess adjuvant properties when combined with DNA vaccines. We showed that B. subtilis spores promoted the activation of dendritic cells in vitro and induced migration of pro-inflammatory cells after parenteral administration to mice. Likewise, co-administration of spores with a DNA vaccine encoding the human papillomavirus type 16 (HPV-16) E7 protein enhanced the activation of antigen-specific CD8(+) T cell responses in vivo. Mice immunized with the DNA vaccine admixed with spores presented a protective immunity increase to previously implanted tumor cells, capable of expressing HPV-16 oncoproteins. Finally, we observed that the adjuvant effect can vary accordingly to the number of co-administered spores which may be ascribed with the ability to induce. Collectively, the present results demonstrate for the first time that B. subtilis spores can also confer adjuvant effects to DNA vaccines. (C) 2015 Elsevier Ltd. All rights reserved.
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