期刊
ARCHIVES OF VIROLOGY
卷 161, 期 3, 页码 731-734出版社
SPRINGER WIEN
DOI: 10.1007/s00705-015-2716-4
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资金
- National Natural Science Foundation of China [31160360]
- Program for Innovative Research Team (in Science and Technology) in University of Yunnan Province
- Opening Fund of Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education [KF201501]
A double-stranded RNA (dsRNA), PN-S, isolated from Panax notoginseng was sequenced and characterized. dsRNA PN-S was determined to be 5003 base pairs (bp) in length and to have a genome organization similar to that of totiviruses. The two deduced proteins encoded by dsRNA PN-S have the highest amino acid (aa) sequence identity to CP and RNA-dependent RNA polymerase (RdRp) of black raspberry virus F (BrVF), a tentative member of the genus Totivirus, family Totiviridae, at 39 % and 54 %, respectively. In a phylogenetic tree based on an alignment of the RdRp amino acid sequences of dsRNA PN-S and selected totiviruses, dsRNA PN-S formed a monophyletic clade with dsRNA viruses belonging to the genus Totivirus. These data suggest that dsRNA PN-S may represent the genome of a member of a novel species of the genus Totivirus, family Totiviridae, for which we propose the name Panax notoginseng virus A (PnVA).
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