期刊
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
卷 15, 期 47, 页码 20510-20516出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cp52621f
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资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- University of Waterloo
- CIHR DSECT
- Canada Foundation for Innovation
- Ontario Research Fund (CFI/ORF)
In this study, the transfection ability and cytotoxicity of a series of phytanyl substituted gemini surfactants, rationally designed and synthesized in an attempt to create cationic surfactants that will improve transfection efficiencies of non-viral vectors was evaluated in OVCAR-3 cells at the charge ratios (N+/P-) of 2 : 1, 5 : 1, and 10 : 1. Particle sizes, zeta potentials, and small angle X-ray scattering (SAXS) profiles were also determined. For each gemini surfactant complex, the transfection efficiency and cytotoxicity are observed to go through a more or less well-evidenced maximum, occurring at different values of the charge ratio (N+/P-), depending on the surfactant structure. Considering both results of in vitro transfection efficiency and cytotoxicity, the optimal charge ratio to formulate the complexes containing phy-3-m was found to be 5 : 1. The particle size decreased, while zeta potential increased with increasing N+/P-. Comparing particle size and zeta potential with transfection efficiency, no correlation between size/zeta potential and transfection ability was observed. Analysis of SAXS profiles indicates that the ability of phy-3-m delivery system to adopt multiple phases correlated well with their higher transfection efficiency in OVCAR-3 cells.
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