期刊
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
卷 15, 期 15, 页码 5379-5385出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2cp43155f
关键词
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资金
- National Research Foundation of Korea [R11-2008-0061852, K20904000004-11A0500-00410]
- Nano Material Technology Development Program through the National Research Foundation of Korea
- Ministry of Education, Science and Technology [2012035286]
A novel SERS-based sandwich immunoassay using DNA aptamers, silica-encapsulated hollow gold nanospheres (SEHGNs) and a gold-patterned microarray was developed for sensitive detection of VEGF (vascular endothelial growth factor) angiogenesis protein markers. Here, a DNA aptamer conjugated to SEHGN was used as a highly reproducible SERS-encoding nanoprobe, and a hybrid microarray including hydrophilic gold wells and other hydrophobic areas was used as a SERS substrate. Target specific DNA aptamers that fold into a G-quadruplex structure were used as a target recognition unit instead of VEGF antibodies. The detection sensitivity was increased by 2 or 3 orders of magnitude over the conventional ELISA method. In particular, the dynamic concentration range was 3 or 4 orders of magnitude greater than that of conventional ELISA. The results demonstrate that this sensing strategy using DNA aptamers is a powerful platform for the design of novel immune-sensors with high performance. In particular, SERS-based detection using SEHGNs provides great promise for highly sensitive biomarker sensing with unprecedented advantages.
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