期刊
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
卷 14, 期 13, 页码 4355-4358出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2cp40219j
关键词
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资金
- Australian Research Council
- Israel Science Foundation (ISF)
- Harold Perlman family
The pulse DEER (Double Electron-Electron Resonance) technique is frequently applied for measuring nanometer distances between specific sites in biological macromolecules. In this work we extend the applicability of this method to high field distance measurements in a protein assembly with mixed spin labels, i.e. a nitroxide spin label and a Gd3+ tag. We demonstrate the possibility of spectroscopic selection of distance distributions between two nitroxide spin labels, a nitroxide spin label and a Gd3+ ion, and two Gd3+ ions. Gd3+-nitroxide DEER measurements possess high potential for W-band long range distance measurements (6 nm) by combining high sensitivity with ease of data analysis, subject to some instrumental improvements.
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