期刊
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
卷 13, 期 2, 页码 530-539出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0cp01062f
关键词
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资金
- Ministerio de Ciencia e Innovacion [CTQ2008-02403/BQU]
- Generalitat de Catalunya [2009SGR409]
We present here a theoretical study of the phosphoryl transfer catalytic mechanism of protein kinase A, which is the best known member of the large protein kinase family. We have built different theoretical models of the complete PKA-Mg-2-ATP-substrate system to explore the two most accepted reaction pathways, using for the first time in a reaction mechanism theoretical study, the heptapeptide substrate Kemptide, which is relevant for its high efficiency and small size. The effect of the protein configuration, as modeled by two different X-ray structures with different phosphorylation states and degrees of flexibility, has been analyzed. The results indicate that the environmental conditions can influence the availability of the pathways and thus the choice of the mechanism to be followed. In addition, the roles of the two active site conserved residues, Asp166 and Lys168, have been analyzed for each reaction mechanism.
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