期刊
PHOTOSYNTHETICA
卷 48, 期 3, 页码 409-416出版社
ACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY
DOI: 10.1007/s11099-010-0053-0
关键词
antisense transgenic plants; chilling stress under low irradiance; photoinhibition; tomato; zeaxanthin epoxidase gene
资金
- State Key Basic Research and Development Plan of China [2009CB118500]
- Natural Science Foundation of China [30871458]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT0635]
- Specialized Research Fund for the Doctoral Program of Higher Education [200804340008]
A tomato (Lycopersicon esculentum Mill.) zeaxanthin epoxidase gene (LeZE) was isolated and antisense transgenic tomato plants were produced. Northern, southern, and western blot analyses demonstrated that antisense LeZE was transferred into the tomato genome and the expression of LeZE was inhibited. The ratio of (A+Z)/(V+A+Z) in antisense transgenic plants was maintained at a higher level than in the wild type (WT) plants under high light and chilling stress with low irradiance. The value of non-photochemical quenching (NPQ) in WT and transgenic plants was not affected during the stresses. The oxidizable P700 and the maximal photochemical efficiency of PSII (F-v/F-m) in transgenic plants decreased more slowly at chilling temperature under low irradiance. These results suggested that suppression of LeZE caused zeaxanthin accumulation, which was helpful in alleviating photoinhibition of PSI and PSII in tomato plants under chilling stress.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据