期刊
PHOTOSYNTHETICA
卷 46, 期 3, 页码 441-451出版社
ACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY
DOI: 10.1007/s11099-008-0076-y
关键词
biotic stress; Nicotiana benthamiana; non-photochemical quenching; Pepper mild mottle virus
资金
- Spanish Ministry of Science and Education [BIO2004-04968-C02-02]
- CSIC/Academy if Sciences of the Czech Republic [2003CZ0011, 2004CZ0016]
- Czech Ministry of Education, Sports, and Youth [MSM6007665808]
- Academy of Sciences of the Czech Republic [AVOZ60870520]
We compared by chlorophyll (Chl) fluorescence imaging the effects of two strains of the same virus (Italian and Spanish strains of the Pepper mild mottle virus - PMMoV-I and-S, respectively) in the host plant Nicotiana benthamiana. The infection was visualized either using conventional Chl fluorescence parameters or by an advanced statistical approach, yielding a combinatorial set of images that enhances the contrast between control and PMMoV-infected plants in the early infection steps. Among the conventional Chl fluorescence parameters, the non-photochemical quenching parameter NPQ was found to be an effective PMMoV infection reporter in asymptomatic leaves of N. benthamiana, detecting an intermediate infection phase. The combinatorial imaging revealed the infection earlier than any of the standard Chl fluorescence parameters, detecting the PMMoV-S infection as soon as 4 d post-inoculation (dpi), and PMMoV-I infection at 6 dpi; the delay correlates with the lower virulence of the last viral strain.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据