3.9 Article

670nm Laser Light and EGCG Complementarily Reduce Amyloid-beta Aggregates in Human Neuroblastoma Cells: Basis for Treatment of Alzheimer's Disease?

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PHOTOMEDICINE AND LASER SURGERY
卷 30, 期 1, 页码 54-60

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MARY ANN LIEBERT, INC
DOI: 10.1089/pho.2011.3073

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  1. Helmholtz Alliance for Mental Health in an Ageing Society (HelMA)
  2. German Research Foundation (DFG) [BI 1409/2]
  3. German Federal Ministry of Education (BMBF) [01GS08132]

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Objective: The aim of the present study is to present the results of in vitro experiments with possible relevance in the treatment of Alzheimer's disease (AD). Background Data: Despite intensive research efforts, there is no treatment for AD. One root cause of AD is the extra- and intracellular deposition of amyloid-beta (Ab) fibrils in the brain. Recently, it was shown that extracellular Ab can enter brain cells, resulting in neurotoxicity. Methods: After internalization of A beta(42) into human neuroblastoma (SH-EP) cells, they were irradiated with moderately intense 670-nm laser light (1000 Wm(-2)) and/or treated with epigallocatechin gallate (EGCG). Results: In irradiated cells, A beta(42) aggregate amounts were significantly lower than in nonirradiated cells. Likewise, in EGCG-treated cells, A beta(42) aggregate amounts were significantly lower than in non-EGCG-treated cells. Except for the cells simultaneously laden with A beta(42) and EGCG, there was a significant increase in cell numbers in response to laser irradiation. EGCG alone had no effect on cell proliferation. Laser irradiation significantly increased ATP levels in A beta(42)-free cells, when compared to nonirradiated cells. Laser-induced clearance of A beta(42) aggregates occurred at the expense of cellular ATP. Conclusions: Irradiation with moderate levels of 670-nm light and EGCG supplementation complementarily reduces Ab aggregates in SH-EP cells. Transcranial penetration of moderate levels of red to near-infrared (NIR) light has already been amply exploited in the treatment of patients with acute stroke; the blood-brain barrier (BBB) penetration of EGCG has been demonstrated in animals. We hope that our approach will inspire a practical therapy for AD.

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