期刊
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
卷 9, 期 1, 页码 52-59出版社
ELSEVIER
DOI: 10.1016/j.pdpdt.2011.08.006
关键词
Photodynamic therapy; Zinc phthalocyanines; Toxicity; Lung cancer; Colon cancer
类别
资金
- University of Johannesburg
- National Research Foundation of South Africa
- Council for Scientific and Industrial Research of South Africa
Background: Photodynamic therapy (PDT) is a therapeutic modality used for treating cancerous cells. It has been previously shown that mixed sulfonated metallophthalocyanine complex, zinc sulfophthalocyanine (ZnPcSmix) is effective in destroying lung cancer cells. This study aimed to determine subcellular localization of ZnPcSmix and its effect on two cancer cell lines. Methods: ZnPcSmix was activated at a wavelength of 680 nm with 5 J/cm(2). Colon (DLD-1) and lung (A549) cancer cell lines were used. Subcellular localization of ZnPcSmix was determined by fluorescence microscopy. Toxicity of PS alone and combination of light and PS (PDT) was determined by cell morphology, viability, proliferation and cytotoxicity. Cells which received no irradiation (0 J/cm(2)), irradiation alone (5 J/cm(2)) or treated with PS alone (no irradiation) served as controls. Results: ZnPcSmix localized in both lysozomes and mitochondria in both A549 and DLD-1 cells. A549 cells treated with PDT showed a significant decrease in viability and proliferation in all PS concentrations used, while in DLD-1 cells a significant decrease was seen with concentrations of 10, 20 and 40 mu M. In absence of light, ZnPcSmix did not result in cellular toxicity in A549 cells whereas in DLD-1 cells it resulted in a reduction in cell proliferation only at a concentration of 40 mu M. Conclusion: ZnPcSmix was effective in inducing cell death in both cell lines when localized in vital organelles such mitochondria and lysozomes which are essential for cell functioning. Photoactivated ZnPcSmix affected the cells at different concentration and yielded good therapeutic results in vitro. (c) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据