Effect of photodynamic therapy and endostatin on human glioma xenografts in nude mice

The aim of this study was to investigate the effect of endostar (a recombinant human endostatin) and photodynamic therapy (PDT) on gliomas. To establish glioma xenografts, human U251 glioma cells were injected into the brain of nude mice. Mice with MRI-confirmed glioma received hematoporphyrin monomethyl ether (HMME)-mediated PDT, daily injection of endostar or their combination, respectively. After treatment, tumor volume, the expression of HIF-1 alpha, VEGF-A and apoptosis marker, and animal survival were examined. PDT and endostar treatment can prolong survival. Changes in induction of apoptosis, tumor growth and survival were more significant in PDT + endostar group. After PDT, HIF-1 alpha and VEGF-A expressions were markedly increased. After endostar treatment, HIF-1 alpha and VEGF-A expressions were significantly reduced. PDT in combination with endostar can significantly inhibit the growth of glioma xenografts. This approach may represent a promising strategy in the treatment of glioma. (C) 2011 Elsevier B.V. All rights reserved.