期刊
PHOTOCHEMISTRY AND PHOTOBIOLOGY
卷 90, 期 5, 页码 1211-1213出版社
WILEY-BLACKWELL
DOI: 10.1111/php.12283
关键词
-
资金
- National Cancer Institute, National Institutes of Health [CA23378]
When the initial effect of photodynamic therapy (PDT) involves mitochondrial photodamage, an early effect is loss of the mitochondrial membrane potential ((m)). Using murine hepatoma 1c1c7 cells and a photosensitizing agent known to target mitochondria, we examined loss of (m), initiation of apoptosis and loss of viability as a function of time and light dose. There was a correlation between loss of viability and the rapid disappearance of (m), as detected by the potential-sensitive probe Mitotracker Orange (MTO). Loss of (m) was, however, reversible even with a substantial loss of viability. Unless there was a supralethal level of photodamage, 1c1c7 cells recovered their mitochondrial membrane potential, even if the cell population was on the pathway to apoptosis and cell death. These results indicate that when mitochondria are the initial PDT target, a qualitative estimate of photokilling can be provided by assessing the initial loss of (m).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据