期刊
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
卷 368, 期 1616, 页码 -出版社
ROYAL SOC
DOI: 10.1098/rstb.2012.0325
关键词
Dehalobacter; organohalide respiration; metabolism; genome; reductive dehalogenase
类别
资金
- The Netherlands Genomics Initiative
- European Community's Seventh Framework Programme (FP7) through the Ecogenomics project [211684]
- US Department of Energy's Office of Science, Biological and Environmental Research Program
- University of California, Lawrence Berkeley National Laboratory [DE-AC02-05CH11231]
- University of California, Lawrence Livermore National Laboratory [DE-AC52-07NA27344]
- University of California, Los Alamos National Laboratory [DE-AC02-06NA25396]
- Swiss National Science Foundation (SNSF) in frame of the SNF project [31003A_138114]
- European Community's Seventh Framework Programme (FP7) through the BACSIN project [211684]
- Swiss National Science Foundation (SNF) [31003A_138114] Funding Source: Swiss National Science Foundation (SNF)
Dehalobacter restrictus strain PER-K23 is an obligate organohalide respiring bacterium, which displays extremely narrow metabolic capabilities. It grows only via coupling energy conservation to anaerobic respiration of tetra- and trichloroethene with hydrogen as sole electron donor. Dehalobacter restrictus represents the paradigmatic member of the genus Dehalobacter, which in recent years has turned out to be a major player in the bioremediation of an increasing number of organohalides, both in situ and in laboratory studies. The recent elucidation of the D. restrictus genome revealed a rather elaborate genome with predicted pathways that were not suspected from its restricted metabolism, such as a complete corrinoid biosynthetic pathway, the Wood-Ljungdahl (WL) pathway for CO2 fixation, abundant transcriptional regulators and several types of hydrogenases. However, one important feature of the genome is the presence of 25 reductive dehalogenase genes, from which so far only one, pceA, has been characterized on genetic and biochemical levels. This study describes a multi-level functional genomics approach on D. restrictus across three different growth phases. A global proteomic analysis allowed consideration of general metabolic pathways relevant to organohalide respiration, whereas the dedicated genomic and transcriptomic analysis focused on the diversity, composition and expression of genes associated with reductive dehalogenases.
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