期刊
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
卷 368, 期 1616, 页码 -出版社
ROYAL SOC
DOI: 10.1098/rstb.2012.0249
关键词
reductive dehalogenation; reductive dehalogenase-homologous gene; substrate-induced gene expression; subseafloor biosphere; biogeochemical carbon and halogen cycle
类别
资金
- Strategic Fund for Strengthening Leading-Edge Research and Development by the Japan Society for the Promotion of Science (JSPS)
- Funding Programme for Next Generation World-Leading Researchers by the Japan Society for the Promotion of Science (JSPS)
- Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)
- JAMSTEC Multidisciplinary Research Promotion Award
- Academy of Finland [122394]
- Finnish Funding Agency for Technology and Innovation grant [40149/07]
- Osk Huttunen's Foundation grant
- Finnish Cultural Foundation grant
- [24651018]
- [21780085]
- Academy of Finland (AKA) [122394, 122394] Funding Source: Academy of Finland (AKA)
- Grants-in-Aid for Scientific Research [24687004, 24651018] Funding Source: KAKEN
Halogenated organic matter buried in marine subsurface sediment may serve as a source of electron acceptors for anaerobic respiration of subseafloor microbes. Detection of a diverse array of reductive dehalogenase-homologous (rdhA) genes suggests that subseafloor organohalide-respiring microbial communities may play significant ecological roles in the biogeochemical carbon and halogen cycle in the subseafloor biosphere. We report here the spatial distribution of dehalogenation activity in the Nankai Trough plate-subduction zone of the northwest Pacific off the Kii Peninsula of Japan. Incubation experiments with slurries of sediment collected at various depths and locations showed that degradation of several organohalides tested only occurred in the shallow sedimentary basin, down to 4.7 metres below the seafloor, despite detection of rdhA in the deeper sediments. We studied the phylogenetic diversity of the metabolically active microbes in positive enrichment cultures by extracting RNA, and found that Desulfuromonadales bacteria predominate. In addition, for the isolation of genes involved in the dehalogenation reaction, we performed a substrate-induced gene expression screening on DNA extracted from the enrichment cultures. Diverse DNA fragments were obtained and some of them showed best BLAST hit to known organohalide respirers such as Dehalococcoides, whereas no functionally known dehalogenation-related genes such as rdhA were found, indicating the need to improve the molecular approach to assess functional genes for organohalide respiration.
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