4.1 Article

In vitro antiproliferative/cytotoxic activity of 2,3′-biindole against various cancer cell lines

期刊

TURKISH JOURNAL OF BIOLOGY
卷 39, 期 1, 页码 15-22

出版社

Tubitak Scientific & Technological Research Council Turkey
DOI: 10.3906/biy-1402-60

关键词

2,3 '-Biindole; anticancer; cytotoxicity; apoptosis; antitopoisomerase; C6 cell line; HeLa cell line; HT29 cell line

类别

资金

  1. Kirikkale University Research Fund [2012/122]

向作者/读者索取更多资源

2,3'-Biindole (2) was synthesized via bromination of indole (1) with molecular bromine and underwent simultaneous dimerization. Antiproliferative and cytotoxic activity of 2 was investigated in vitro on C6 (rat brain tumor), HeLa (human cervix carcinoma), and HT29 (human colon carcinoma) cells lines by using BrdU cell proliferation ELISA and lactate dehydrogenase (LDH) assays. In contrast to 5-fluorouracil (5-FU), 2,3'-biindole (2) significantly inhibited proliferation of HeLa and HT29 cell lines. According to LDH assay, the cytotoxicity of compound 2 was low on HT29 cell lines and high on HeLa and C6 cell lines. Moreover, 2 did not cause any DNA laddering on the DNA of tested cells; therefore, it is suggested that the mechanism of action of this compound may not involve apoptosis. In addition, 2 inhibited relaxation of supercoiled plasmid DNA by topoisomerase activity. Results of the present study indicates that biindole (2) may have promising anticancer and antitopoisomerase potential with an unknown mechanism of action.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.1
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据