期刊
PHILOSOPHICAL MAGAZINE
卷 92, 期 19-21, 页码 2648-2661出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786435.2012.670734
关键词
membrane protein; X-ray crystallography; detergent; lipid; efflux pump
类别
资金
- NIH [R01GM086431]
Integral membrane proteins comprise approximately 30% of the sequenced genomes, and there is an immediate need for their high-resolution structural information. Currently, the most reliable approach to obtain these structures is X-ray crystallography. However, obtaining crystals of membrane proteins that diffract to high resolution appears to be quite challenging, and remains a major obstacle in structural determination. This brief review summarizes a variety of methodologies for use in crystallizing these membrane proteins. Hopefully, by introducing the available methods, techniques, and providing a general understanding of membrane proteins, a rational decision can be made about now to crystallize these complex materials.
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